The interface to the eBURST application
is split into three sections –
The Profiles window.
The Analysis window.
The Diagram window
Each of these are accessible via ‘TABS’ within
the interface, and each of the windows
contains menu options, a main window and
a panel containing context sensitive functions
based on which window you are viewing – see
The Profiles window
The data used by eBURST is a list of STs and their allelic profiles. Data can
be entered by ‘cut and paste’ and should be tab-delimited (or space-delimited)
text, each line having the ST number followed by the allele numbers at the
seven MLST loci (in the standard order of loci for the MLST scheme).
An example of correctly formatted input
text is shown below:
eBURSTv3 is integrated
within the mlst.net databases and can
be launched after querying the database
to examine subsets of a population,
or can be launched to view the entire
population of a particular species.
The option also exists
to combine your own data, added by ‘cut
and paste’ or upload, with all
of the data that is in a MLST database.
On clicking an eBURST button, the output
from the database query, the whole database,
or your data is sent to eBURST.
HINT – you may
wish to use eBURST to compare your own
isolates with all of the isolates of
the species in the MLST database, but
your MLST data may have alleles and/or
STs that are not in the MLST database.
These isolates can still be analysed
by eBURST as they can be given temporary
allele numbers (we suggest numbers above
1000 to avoid any confusion with the
official allele numbers) and temporary
ST numbers (we suggest greater than
10,000). Note that these numbers are
just to allow you to analyse your data
using eBURST and should NEVER be used
in any publication (new alleles and
STs should be submitted to the curator
of the appropriate MLST database for
official allele number and ST number
On submitting the data,
eBURST will be launched and the data
will appear in the ‘Profiles’ window.
Once the data is loaded it can be sorted
by ST number or by allelic profile using
the ‘Profiles Menu’. Sorting
by allelic profile is not optimised
and sorts by the allele number at the
first locus, then at the second locus,
The ‘Open’ button
in the ‘Profiles Menu’ allows
a file to be uploaded and this option,
or ‘cut and paste’ within
the MLST website, allows any MLST data
to be analysed by eBURST. The uploaded
data will replace any data already in
the Profiles window. Uploaded files
must be correctly formatted text files,
as in the example above.