Click for Instructions


Defining groups
Identifying the founder of a group and the patterns of evolutionary descent
Estimating the level of confidence in the predicted founding ST
Building up the eBURST diagram
Exploring alternative patterns of descent
Using additional data to optimise patterns of descent

Using eBURST

The Profiles window
The Analysis window
The Diagram window
Subgroups and Subgroup founders
Comparing two datasets
Manipulaing and editing the display
Using eBURSTv3 standalone
Software requirements

click here to download the entire eBURSTv3 readme as .pdf


  • Feil EJ, Li BC, Aanensen DM, Hanage WP, Spratt BG. 2004 eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J Bacteriol. Mar;186(5):1518-30
  • Spratt BG, Hanage WP, Li B, Aanensen DM and Feil EJ. (2004) Displaying the relatedness among isolates of bacterial species -- the eBURST approach. FEMS Microbiol Lett. Dec 15;241(2):129-34

  • eBURSTv3 has been developed and is hosted at Imperial College London