The Profiles window
The data used by eBURST is a list of STs and their allelic
profiles. Data can be entered by ‘cut and paste’ and
should be tab-delimited (or space-delimited) text, each
line having the ST number followed by the allele numbers
at the seven MLST loci (in the standard order of loci for
the MLST scheme).
An example of correctly formatted input text is shown below:
In many cases eBURST will be
used on output produced by querying a MLST database, or will
input the whole of a MLST database.
The option also exists to combine your own data, added
by ‘cut and paste’, with all of the data that
is in a MLST database. On clicking an eBURST button, the
output from the database query, the whole database, or ‘cut
and paste’ is sent to the eBURST applet.
HINT – you may wish to use eBURST to compare your
own isolates with all of the isolates of the species in the
MLST database, but your MLST data may have alleles and/or
STs that are not in the MLST database. These isolates can
still be analysed by eBURST as they can be given temporary
allele numbers (we suggest numbers above 1000 to avoid any
confusion with the official allele numbers) and temporary
ST numbers (we suggest greater than 10,000). Note that these
numbers are just to allow you to anayse your data using eBURST
and should not be used in any publication (new alleles and
STs should be submitted to the curator of the appropriate
MLST database for official allele number and ST number assignments).
On submitting the data, the eBURST Java™ applet will
be launched and the data will appear in the applet ‘Profiles’ window.
Once the data is loaded into the applet it can be sorted
by ST number or by allelic profile using the ‘Profiles
Panel’. Sorting by allelic profile is not optimised
and sorts by the allele number at the first locus, then at
the second locus, etc.
The ‘Open’ button in the ‘Profiles Panel’ of
the applet allows a file to be uploaded and this option,
or ‘cut and paste’ within the MLST website, allows
any MLST data to be analysed by eBURST. The uploaded data
will replace any data already in the Profiles window. Uploaded
files must be correctly formatted text files, as in the example